Long-read Transcriptomics

REGISTER HERE

Date:

11 - 13 November 2020

10am - 3pm each day

Venue:

Online

Places:

24 for each workshop

You will be contacted by our finance team for full payment. Once payment is made, your place will be confirmed and full details sent by our training team.

Registration fee:

£150 - University of Edinburgh staff/students
£158 - Other university or registered charity staff/students
£195 - Industrial researchers

Information:

Contact our training team

 

Overview

The recent development of high throughput long read RNA sequencing promises a new age of transcriptome exploration. Accurate high throughput long read RNA sequencing now has the potential to investigate genes that were previously undetectable.

This exciting new course aims to introduce the principles and practice of long-read transcriptomic anlysis using the cutting edge software TAMA (https://github.com/GenomeRIK/tama). 

Who this course is for

This course is intended for researchers who need to analyse genomic data in order to call genomic variants. Aside from a basic understanding of molecular biology, attendees must have a working knowledge of how to use the Linux BASH command line - our 1-day "Linux for bioinformatics" course is a suitable background.

 


Instructor

Richard Kuo - University of Edinburgh 

Workshop format

The workshop consists of presentations and hands-on tutorials.

 


Topics covered

By the end of the course students will have covered:

  • Introduction to Pacific Bioscience and Oxford Nanopore Technologies RNA sequencing
  • Long read data pre-processing (Pacbio and Nanopore)
  • Transcriptome assembly (TAMA collapse)
  • Merging Transcriptomes (TAMA Merge)
  • Linking gene models to known proteins (TAMA ORF/NMD Pipeline)
  • Transcriptome filtering