Illumina mRNA Library Preparation Workshop

Sign up here! (UoE staff/students only)

 

Date:

14-15 June 2022

Day 1: 9.30am - 5.30pm (gmt)
Day 2: 10am - 16.00pm (gmt)

Venue:

Edinburgh Genomics, Ashworth Laboratory (ground floor), The Kings Buildings Campus, Charlotte Auerbach Road, EH9 3FL

Places:

12 for each workshop

You will be contacted by our finance team for full payment. Once payment is made, your place will be confirmed and full details sent by our training team.

Registration fee:

£190 - University of Edinburgh staff/students
 

Information:

Contact our training team

 

Edinburgh Genomics and Illumina are excited to bring you a new lab-based workshop designed for genomicists looking to make their own high-quality NGS libraries ready for sequencing. This workshop will consist of both in-person presentations and hands-on lab work taking you through the theory and techniques of mRNA library preparation.  

Illumina, the forerunners of innovation in next-generation sequencing, will bring their unrivalled expertise to guide participants in the use of their own mRNA prep kits, whilst Edinburgh Genomics will provide a fully equipped genomics laboratory along with expert technicians to help with protocols.  

 

Instructors

  • Cathlene Eland – Illumina 
  • Laura Ingram - Illumina
  • Edinburgh Genomics lab team
     

Workshop format

The workshop consists of presentations and hands-on tutorials.
 

Who should attend

This course is primarily aimed at University of Edinburgh researchers and technicians who have used or plan to use Edinburgh Genomics sequencing facilities and plan to make their own libraries for sequencing in the future. 

It is suitable for postgraduates, technicians, and PIs, using NGS data in their research and who have some experience working in a molecular wet lab prior to the start of the course. 

Covered topics

Day 1

  • Introduction to Illumina sequencing and review of Illumina stranded mRNA workflow 

  • Purification and fragmentaton of mRNA 

  • Synthesis of stranded cDNA

  • Adenylation of 3' ends 

  • Ligation of anchors 

  • Fragment clean up 

    Day 2

  • Amplification and clean up of Libraries

  • Review and discussion

  • Sequencing lab tour

  • Pooling and sequencing of prepared libraries (EdGe)