Cross-species inference of long non-coding RNAs greatly expands the ruminant transcriptome

Bush, S. J., Muriuki, C., McCulloch, M. E. B., Farquhar, I. L., Clark, E. L. & Hume, D. A.
Genetics Selection Evolution
Journal publication date: 

BACKGROUND: mRNA-like long non-coding RNAs (lncRNAs) are a significant component of mammalian transcriptomes, although most are expressed only at low levels, with high tissue-specificity and/or at specific developmental stages. Thus, in many cases lncRNA detection by RNA-sequencing (RNA-seq) is compromised by stochastic sampling. To account for this and create a catalogue of ruminant lncRNAs, we compared de novo assembled lncRNAs derived from large RNA-seq datasets in transcriptional atlas projects for sheep and goats with previous lncRNAs assembled in cattle and human. We then combined the novel lncRNAs with the sheep transcriptional atlas to identify co-regulated sets of protein-coding and non-coding loci.